Griffiths, R.; Pywell, R.F.; Peyton, J.; Nuttall, P.; Sarlej, R.; Thomson, B.

Operational taxonomic units for soil eukaryotes in the rivers Wolf and Tamar catchments

Name: Operational taxonomic units for soil eukaryotes in the rivers Wolf and Tamar catchments
Published: 2014-05-01
License: https://eidc.ceh.ac.uk/licences/OGL/plain

https://doi.org/10.5285/4bf6228f-ce3d-449e-9438-c4b5c8291256 Cite this dataset

This dataset contains operational taxonomic units for soil eukaryotes from the Wolf and Tamar catchments . A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken. The exact location of each sample site was determined using a Garmin GPS12. Soil samples were kept in the cold and removed to the laboratory for analyses.

Publication date: 2014-05-01

Format

Comma-separated values (CSV)

Spatial information

Study area
Spatial representation type: Tabular (text)
Spatial reference system: OSGB 1936 / British National Grid

Temporal information

Temporal extent: 2013-10-01 to 2014-03-20

Provenance & quality

Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

This dataset is included in the following collections

Molecular analyses of soils and epilithon samples from the Tamar catchment

Correspondence/contact details

Point of Contact

UK Centre for Ecology & Hydrology

Maclean Building, Benson Lane, Crowmarsh Gifford
Wallingford
Oxfordshire
OX10 8BB
UNITED KINGDOM

enquiries@ceh.ac.uk